Intended Use

• The 2X qPCR Master Mix is used for real-time qualitative and quantitative qPCR with TaqMan probe.

• The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probe and DNA template.

Specification

• The kit is designed for singleplex qPCR with TaqMan probe.

• This kit uses Taq-Probe DNA polymerase specially engineered for TaqMan probe, which increased 5’ to 3’ exonuclease efficiency producing S-shaped curve.

• The preferable PCR product size is ≤150 bp.

User Manual

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