Intended Use

• The 2X Multiplex qPCR Master Mix is used for real-time qualitative and quantitative multiplex qPCR with up to four TaqMan probes.

• The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and  templates.

Specification

• The kit is designed for multiplex qPCR with TaqMan probes.

• The kit uses Taq-Probe DNA polymerase specially engineered for TaqMan probes, which increases 5’ to 3’ exonuclease efficiency producing S-shaped curve.

• Up to four pairs of gene-specific primers can be applied in one reaction.

• The preferable PCR product size is ≤150 bp.

User Manual

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