Thermophilic Reverse Transcriptase:

1) Origin: It originates from a type A thermophilic DNA polymerase.

2) Optimum temperature: 60-62°C.

2) Highly efficient: ≤1 unit per 20 ul reaction,

3) Heat inactivated: ≥90°C for 1 min

4) Detection limit: as low as single digit copies of target RNA template

5) Particularly suitable to one-step realtime-fluorescence RT-PCR

Pyrophosphorolysis Activated Polymerization (PAP)

PAP is a next generation of nucleic acid amplification technology. Unlike traditional PCR, this PAP introduces a new principle in which two distinct reactions (pyrophosphorolysis and polymerization) are serially coupled by a polymerase using a blocked primer.  

Double Layer Column Technology

Double layer column is based on next generation of nucleic acid extraction technology. Unlike the conventional silica technology, it introduces a new principle in which two distinct membranes are serially coupled within a column: the first DEAE anion exchange membrane and the second silica membrane.