Cat. RT-PCR 140, Thermophilic Reverse Transcriptase
Cat. RT-PCR 140, Thermophilic Reverse Transcriptase
Intended use
This reverse transcriptase or RNA-dependent DNA polymerase is used for real-time fluorescent RT-PCR with either SYBR Green dye or TaqMan probe.
Specification
This reverse transcriptase originates from a thermophilic type A polymerase (US patent pending).
This RTase works best at 60-62°C.
It is easily heat-inactivated at ≥90°C for 1 min.
The RTase efficiently synthesizes a complementary DNA on RNA template from a gene-specific primer, ≤1 unit per 20 μL reaction.
The enzyme reverse-transcribes single digit copies of target RNA molecules consistently.
It is particularly suitable to one-step real-time qualitative and quantitative RT-PCR.
Order Selections:
The standard shipping box measures 11X9X10 inches and it contains the cold gel pack. Each box can hold up to 6 orders. We ship using FedEx overnight express.