Intended Use

• The Master Mix is used for real-time qualitative and quantitative RT-PCR from extraction-free RNA sample of tissue cultured cells.

• The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.

Specification

• Using Thermophilic Reverse Transcriptase and Taq Fast DNA polymerase, the master mix contains extra redundant enzyme amounts, optimized to overcome endogenous inhibition in extraction-free RNA samples.

• For the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which originates from a thermophilic type A polymerase.

• This RTase works best at 60-62 °C.

• It is easily heat-inactivated at ≥90 °C for 1 min.

• The enzyme efficiently synthesizes a complementary DNA on RNA template, 1 unit per 20 μL reaction.

• The RTase reverse-transcribes single digit copies of target RNA molecules consistently.

• The kit also contains engineered Taq-Fast DNA polymerase which extends more than 300 bases with short cycling program.

• The preferable PCR product size is ≤150 bp.

User Manual

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