Intended Use

• The 2X Multiplex Super qPCR Master Mix is used for real-time qualitative and quantitative multiplex qPCR with TaqMan probes for up to four targets.

• The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and  templates.

Specification

• The kit is designed for multiplex qPCR with TaqMan probes.

• The kit uses Super Taq-Probe DNA polymerase II specially engineered for TaqMan probes, which increases 5’ to 3’ exonuclease efficiency  producing S-shaped curve.

• Two to four pairs of gene-specific primers can be applied in one reaction.

• The preferable PCR product size is ≤150 bp.

User Manual

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